Antioxidant Activity and Phytochemical Properties of Methanol,ethanol and Hexane Some Extracts of Alstoniaboonei Leaves

Antioxidant Activity and Phytochemical Properties of Methanol,ethanol and Hexane Some Extracts of Alstoniaboonei Leaves

Content Structure of Antioxidant Activity and Phytochemical Properties of Methanol,ethanol and Hexane Some Extracts of Alstoniaboonei Leaves

The abstract contains the research problem, the objectives, methodology, results, and recommendations

• Chapter one of this thesis or project materials contains the background to the study, the research problem, the research questions, research objectives, research hypotheses, significance of the study, the scope of the study, organization of the study, and the operational definition of terms.
• Chapter two contains relevant literature on the issue under investigation. The chapter is divided into five parts which are the conceptual review, theoretical review, empirical review, conceptual framework, and gaps in research
• Chapter three contains the research design, study area, population, sample size and sampling technique, validity, reliability, source of data, operationalization of variables, research models, and data analysis method
• Chapter four contains the data analysis and the discussion of the findings
• Chapter five contains the summary of findings, conclusions, recommendations, contributions to knowledge, and recommendations for further studies.
• References: The references are in APA
• Questionnaire.  

Abstract On Antioxidant Activity and Phytochemical Properties of Methanol,ethanol and Hexane Some Extracts of Alstoniaboonei Leaves

In traditional setting, Alstoniaboonei is used for the treatment of different ailments, this has been attributed to its rich phytochemical and antioxidant properties.The objective of the study was to evaluate the phytochemical properties and antioxidant activity of the methanol, ethanol and hexane extracts of Alstoniaboonei leaves. Qualitative analysis of phytochemical constituents such as tannins, saponins, cardiac glycosides flavonoids and phenols were carried out. Quantitative analysis of total phenolics, saponins and flavonoids was performed by well-known test protocol. Antioxidant activity reveals the presence of reactive oxygen species which helps to scavenge free radicals that are present in the body.  The in vitro antioxidant activity was studied by diphenyl-2-picryl-hydrazyl (DPPH), ferric reducing antioxidant power (FRAP), reducing power (RP) and ferrous ion metal chelating. Results obtained for phytochemical studies revealed that flavonoids were  highest in ethanol extract(101.6±1.35) while hexane extract (56.6±1.510) had the lowest content. Also phenol was highest in methanol extract (262±4.93) and lowest in ethanol extract (172.7±1.66). Result for total tannins (271±6) and proanthocyanidin (200.7±2.19) indicates that  methanol and ethanol extract had the highest amount while hexane extract  had and the lowest amount. IC₅o values obtained by DPPH for ethanol extracts of Alstoniaboonei (2.362µg/ml), when compared with other extracts, and was significantly (P>0.05) lower than that of the standard vitamin C (12.81µg/ml). Also, the ethanol extract(0.026µg/ml) showed low IC₅₀ value for ferrous ion metal chelating ability when compared with other extract, but this value was non significantly(P>0.05) lower than the standard(4.11µg/ml). Therefore,  Alstoniaboonei has a great potential for use as a natural source of  antioxidant and also help in the treatment of various ailments, or against free radical damage.

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