Studies on the Antimicrobial Properties of Bark and Leaves of Monkey Kola (Cola Millenii) on Some Selected Pathogens

Studies on the Antimicrobial Properties of Bark and Leaves of Monkey Kola (Cola Millenii) on Some Selected Pathogens

Content Structure of Studies on the Antimicrobial Properties of Bark and Leaves of Monkey Kola (Cola Millenii) on Some Selected Pathogens

The abstract contains the research problem, the objectives, methodology, results, and recommendations

• Chapter one of this thesis or project materials contains the background to the study, the research problem, the research questions, research objectives, research hypotheses, significance of the study, the scope of the study, organization of the study, and the operational definition of terms.
• Chapter two contains relevant literature on the issue under investigation. The chapter is divided into five parts which are the conceptual review, theoretical review, empirical review, conceptual framework, and gaps in research
• Chapter three contains the research design, study area, population, sample size and sampling technique, validity, reliability, source of data, operationalization of variables, research models, and data analysis method
• Chapter four contains the data analysis and the discussion of the findings
• Chapter five contains the summary of findings, conclusions, recommendations, contributions to knowledge, and recommendations for further studies.
• References: The references are in APA
• Questionnaire.

Abstract Of Studies on the Antimicrobial Properties of Bark and Leaves of Monkey Kola (Cola Millenii) on Some Selected Pathogens

This study is conducted to analyse the phytochemical component, evaluate the antimicrobial properties of the ethanolic, methanolic and water extracts of Cola millenii  bark and leaves against some clinical Pathogens which are Staphylococcus aureus, Escherichia coli, Salmonella typhi and Candida albicans, to determine the minimum inhibitory concentration, minimum bactericidal concentration(MBC) and minimum fungicidal concentration(MFC). Cola millenii has been used in folk medicine and is a member  of the family Sterculiaceae. The bark and leaves of Cola millenii K Schum were extracted using aqueous(water), ethanolic(organic), methanolic(organic) solvents and were investigated for antimicrobial activity against some pathogenic micro-organisms using agar well diffusion method.  The aqueous extract did not show any significant difference in their activities against the various organisms but the ethanolic extract and methanolic extract had significant activities, ethanolic leaf extract demonstrated the highest activity against  the gram positive  organism(Staphylococcus aureus) tested  with the highest activity(18.00±0.5 mm), and also against the selected test fungi (Candida albicans) with activity (16.00±0.8 mm) zone of inhibition except for Salmonella typhi which did not show any zone of inhibition. Ethanolic bark extract demonstrated the highest activity against  the gram negative organism tested (Escherichia coli) with the highest activity(13.00±0.8 mm), and also against the selected test fungi (Candida albicans) with activity (11.50±0.6 mm) zone of inhibition except for Salmonella typhi which did not show any zone of inhibition. The chloramphenicol and fungisol control showed large zones of inhibition. The minimum inhibitory concentration(MIC) and minimum fungicidal concentration(MFC) of the extract ranges between 125 mg/ml to 250 mg/ml for both the bark and leaves of the plant. The antimicrobial property shown by the plant extract is an evidence of the ethno-medicinal uses of the plant. Cola millenii bark and leaves bioactive component may therefore be extracted and used as antibiotics as the result is comparable with commercially available antibiotics and incorporation of active compounds isolated from the ethanolic extract of Cola millenii into conventional drug preparation`ns can also tackle the challenge posed by drug resistant microorganisms.

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